FARRZYME™ High Avidity anti dsDNA Kit
FARRZYME is a selective EIA assay for the detection of high avidity IgG anti-dsDNA antibodies.
When used in combination with other assays, FARRZYME aids the diagnosis of Systemic Lupus Erythematosus (SLE) and lupus nephritis.
High avidity anti-dsDNA antibodies
Serological detection of anti-dsDNA antibodies can be an important indicator of disease activity in SLE. The association between high avidity serum IgG anti-dsDNA antibodies and renal inflammatory activity has been reported. Patients are at a higher risk of progressing to more severe disease if they have high avidity antibodies compared to those patients who have only lower avidity anti-dsDNA antibodies detectable.1, 2
The selectivity of FARRZYME for high avidity anti-dsDNA antibodies has been clearly demonstrated in the table below.
| Monoclonal Antibody | High Avidity Antibody - 32B9 (9.6 x 10-7Kd) | Low Avidity Antibody - 33H11 (6.6 x 10-7Kd) |
|---|---|---|
| FARRZYME (IU/mL) | Strong Positive | Negative |
| Standard anti-dsDNA EIA (IU/mL) | Strong Positive | Strong Positive |
- Download literature - FARRZYME High Avidity Anti-Double Stranded DNA Assay - MKG382
- Download GERMAN literature - FARRZYME High Avidity Anti-Double Stranded DNA Assay - MKG427
- Download FRENCH literature - FARRZYME High Avidity Anti-Double Stranded DNA Assay - Code MKG389
Calibration
The assay is calibrated in IU/mL against the WHO reference Wo80. A five point calibration curve is generated.
Result Interpretation
| Result | Interpretation |
|---|---|
| < 30 IU/mL | Negative |
| > 30 IU/mL | Positive |
Measuring Range
12.3 - 1000 IU/mL
Sensitivity
12.3 IU/mL
Return to list of Enzyme Immunoassays (EIA)
References
1. Isenberg D., Smeenk R. Clinical laboratory assays for measuring anti-dsDNA antibodies. Where are we now? Lupus 2002;11:797-800
2. Isenberg, D.A., Manson, J.J., Ehrenstein, M.R., Rahman, A. Fifty years of anti-ds DNA antibodies: are we approaching journey's end? Rheumatol 2007;0 kem112v:1-5
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