SPE (SPEP) & IFE for monoclonal gammopathy investigation
Serum Protein Electrophoresis
SPE gel - Tracks
1 & 10 Normal Serum
2, 3, 4 IgG paraproteins
5, 6, 7 IgA paraproteins
8,9 IgM paraproteins
Serum protein electrophoresis (SPE or SPEP) can be used for the initial screening of serum (or concentrated urine) for monoclonal immunoglobulins or M-protein and light chains (Bence Jones protein), aiding in the diagnosis of monoclonal gammopathies.
However, optimal detection of paraproteins is achieved by screening serum samples both with SPE or CZE (Capillary Zone Electrophoresis) and Freelite serum free light chain assays.
Samples exhibiting either abnormal bands by SPE or abnormal levels of free kappa, free lambda or κ/λ ratio by Freelite should be further investigated by immunofixation and quantitation of other specific serum proteins.
Each SPE kit contains reagents sufficient for 100 tests. Each of the 10 gels can be used to run 10 samples.
| Description | Pack | Code |
|---|---|---|
| Serum Protein Electrophoresis kit (SPE) | 10x10 test | XK003 |
Electrophoresis and Immunofixation
IgM Lambda and IgG Kappa monoclonal
immunoglobulins on a MINIFIX IFE gel
Immunofixation electrophoresis (IFE) is a two-stage process combining agarose gel electrophoresis with immuno-precipitation. It has replaced IEP (immunoelectrophoresis) as the method of choice for identification and characterisation of monoclonal immunoglobulins in serum, urine and cerebrospinal fluid. IFE allows ease of interpretation, fast results (less than 2 hours) and has good sensitivity.
Standard IFE
- One patient sample per gel (102 x 77mm)
- Six gel tracks (IgG, IgM, IgA, Kappa, Lambda, TSP [Total serum protein])
MINIFIX™ IFE
- Two patient samples per gel (102 x 77mm)
- 2x Six gel tracks (IgG, IgM, IgA, Kappa, Lambda, TSP)
| Description | Pack | Code |
|---|---|---|
| Immunofixation (IFE) kit | 10 test | XK001 |
| MINIFIX (IFE) kit | 20 test | XK004 |
IFE Components
Individual vials of immunofixation antisera are available for customers who prefer to purchase reagents separately. These products are optimised for use with the IFE (XK002) gel kit which contains the same components as a standard IFE kit but without antisera.
| Description | Pack | Code |
|---|---|---|
| Kappa (Free) | 1.4mL | PX016 |
| Lambda (Free) | 1.4mL | PX018 |
| IgD | 1.4mL | PX013 |
| IgE | 1.4mL | PX014 |
| Complement C3* | 1.4mL | PX023 |
| Fibrinogen* | 1.4mL | PX056 |
| IFE kit without antisera | 10 test | XK002 |
* Research use only
MINIFIX™ is a trademark of The Binding Site Ltd, Birmingham, UK
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